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Figure 1. EGFR is over-expressed in SCCHN cell lines. qRT-PCR was performed to measure ErbB transcript levels in the three SCCHN cell lines, normalized to that of the normal oral <t>epithelial</t> (NOE) cells. Expression fold change was determined by the 22DDCt method. ***p, 0.001; student’s t test. doi:10.1371/journal.pone.0098557.g001
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Figure 1. EGFR is over-expressed in SCCHN cell lines. qRT-PCR was performed to measure ErbB transcript levels in the three SCCHN cell lines, normalized to that of the normal oral <t>epithelial</t> (NOE) cells. Expression fold change was determined by the 22DDCt method. ***p, 0.001; student’s t test. doi:10.1371/journal.pone.0098557.g001
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Figure 1. EGFR is over-expressed in SCCHN cell lines. qRT-PCR was performed to measure ErbB transcript levels in the three SCCHN cell lines, normalized to that of the normal oral <t>epithelial</t> (NOE) cells. Expression fold change was determined by the 22DDCt method. ***p, 0.001; student’s t test. doi:10.1371/journal.pone.0098557.g001
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Figure 1. EGFR is over-expressed in SCCHN cell lines. qRT-PCR was performed to measure ErbB transcript levels in the three SCCHN cell lines, normalized to that of the normal oral epithelial (NOE) cells. Expression fold change was determined by the 22DDCt method. ***p, 0.001; student’s t test. doi:10.1371/journal.pone.0098557.g001

Journal: PloS one

Article Title: Pre-clinical characterization of Dacomitinib (PF-00299804), an irreversible pan-ErbB inhibitor, combined with ionizing radiation for head and neck squamous cell carcinoma.

doi: 10.1371/journal.pone.0098557

Figure Lengend Snippet: Figure 1. EGFR is over-expressed in SCCHN cell lines. qRT-PCR was performed to measure ErbB transcript levels in the three SCCHN cell lines, normalized to that of the normal oral epithelial (NOE) cells. Expression fold change was determined by the 22DDCt method. ***p, 0.001; student’s t test. doi:10.1371/journal.pone.0098557.g001

Article Snippet: The normal oral epithelial (NOE) cell line was maintained in normal human oral epithelial media (Celprogen).

Techniques: Quantitative RT-PCR, Expressing